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While most of the mentioned technologies can also be adapted and used to sequence RNA instead of DNA63,64, directly sequencing proteins remains a challenge. The sequence of amino acids making up a protein is usually deduced from the codons in sequenced DNA or RNA after detection of potentially coding regions called open reading frames (ORFs). Development of methods to directly sequence protein molecules using mass spectrometry was started not very long after Sanger sequencing65 and improved66. New methods are still being developed67 but protein sequencing remains a challenge.
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